Non-Viral Directed Differentiation of hiPSCs Through Single-Step Microfluidic Cell Squeeze® Delivery of Multiple mRNAs Encoding Transcription Factors

Time: 11:30 am
day: Day 2 Track 1 AM


  • Clinically implemented, non-viral Cell Squeeze® technology treatment does not change baseline phenotype or gene expression in iPSCs and supports efficient delivery
  • One-time delivery of a single mRNA transcription factor drives rapid generation of excitatory neurons
  • One-time delivery of six RNA transcription factors, including one saRNA, drives an up to 25,000 fold increase in target genes and generation of dopaminergic neurons